Greater than 2 grams per kg of reference body weight may be an example of too much protein per day, if you want to stay in ketosis. As with most weight loss trials, these studies show that weight loss tends to lessen at the 6-12 month mark. This suggests that they are taking in more calories, more carbs or both. Therefore, we believe most people should maintain low-carb long term to maintain weight loss benefits. Remember that the levels at which carbohydrate and protein exert their effects on the body seem to reflect thresholds, not a percentage of calories.